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. 2023 Aug 28;17:2593–2611. doi: 10.2147/DDDT.S421300

Figure 5.

Figure 5

Identification of the regulation of RHCG levels by HOPX in keratinocytes. (A) Table diagram showing the basic profiles of seven genes with potential regulatory effects on RHCG (HEKn celline, human primary epidermal keratinocytes, data from seven independent datasets). Three of these genes (SUPT6H, HOPX, and BRD4) have a positive regulatory effect on RHCG and four (HNRNPA3, TOPBP1, POGLUT1, and HNRNPK) have a negative regulatory effect on RHCG. pbgene represents perturbation gene. See also Figure S7. (B and C) UMAP plot demonstrating the expression levels of three positively regulating genes (B) and four negatively regulating genes (C) in keratinocytes. See also Figure S8. (D and E) Jitter plots showing the expression level of seven pbgenes and RHCG changing with pseudotime from Cluster 0 to Cluster 1 (D) and Cluster 2 (E). See also Figure S8. (F) Double IF staining images of RHCG and HOPX in the psoriasis sample biopsy. Scale bar, 100 mM. The representative views of co-staining were shown in the enlarged images at right (scale bar, 20 mM). (G) Western blots of RHCG after si-HOPX treatment in HaCaT and HEKn cell lines. **P < 0.01, ***P < 0.001, ****P <0.0001.