Fig 6. Relative expression of AtEFR gene in transgenic banana events.
(A) Reverse-transcriptase (RT)-PCR products corresponding to 100 bp AtEFR gene fragment, (B) RT-PCR products corresponding to Musa 25s gene (internal control), (C) Transcription level of AtEFR in the transgenic bananas as determined using qRT-PCR. Data shown are fold induction relative to the non-transgenic control and were normalized against the internal control gene Musa 25s. Data presented are mean± standard deviation (SD) of three replicate experiments. Bars with different letters are significantly different at p<0.05 according to Fisher’s HSD test. M; 100-base pair marker; T; Transgenic event expressing AtEFR, C; non-transgenic control.
