Figure 2. Secreted protease activity is reduced among sheen positive PA keratitis isolates and can be complemented.

A. Secreted protease activity by PA keratitis isolates. The zone of clearance (mm) on milk plate assay is shown. Each data point indicates the mean zone of clearance for an individual isolate. Medians and IQ ranges are shown. *, p<0.001 by Mann-Whitney. B. Secreted protease activity measured by azocasein and normalized by bacterial density from sterile culture filtrates from the clinical isolate PAC (WT) and isogenic ΔlasR mutant and the mutant with wild-type lasR on a plasmid. The bacteria were grown in LB medium and were harvested at OD₆₀₀=2. Asterisks indicate p<0.01 between indicated groups by ANOVA with Tukey's post-test, n=3, mean and SD are shown.