| pIDN1/3 |
Insertion-duplication plasmid (ErmR) |
Hamilton et al (2001) (24) |
| pKH37 |
Complementation vector (CmR) |
Kohler et al (2007) (25) |
| pKH52 |
pacA point mutation (H329Q) |
Dillard and Hackett (2005) |
| pMRS1 |
mltG deletion constructed in pIDN3, Gibson cloning |
This study |
| pKH189 |
mltG in pTEV5; 6x HIS tag |
This study |
| pKH193 |
mltG in pKLD116; 6x HIS tag + MBP |
This study |
| pKH198 |
mltG complementation; containing mltG in pKH37 (CmR) |
This study |
| pKH209 |
mltG gene block in pIDN3; E213Q point mutant |
This study |
| pTEV5 |
Vector for synthesis of recombinant protein with a N-terminal hexahistidine, removable by tobacco etch virus (TEV) protease |
Rocco et al (2008) (26) |
| pKLD116 |
Vector for synthesis of recombinant protein with a N-terminal hexahistidine and maltose binding protein tag in tandem, removable by TEV. |
Rocco et al (2008) (26) |
| pRS91 |
ltgA point mutant constructed in pIDN1 |
Schaub et al (2016) (28) |
| pRS92 |
ltgD point mutant constructed in pIDN1 |
Schaub et al (2016) (28) |
| |
|
|
| MS11 |
Wildtype Neisseria gonorrhoeae
|
Segal et al (1985) (27) |
| MRS500 |
MS11 transformed with pMRS1; mltG deletion mutant |
This study |
| KH530 |
MS11 transformed with pKH52; pacA point mutation H329Q |
Dillard and Hackett (2005) |
| KH624 |
KH530 transformed with 1291ΔmsbB chromosomal DNA; pacA point mutation H329Q, msbB mutation (KanR) |
|
| KH651 |
MRS500 transformed with pKH198; mltG complemented (CmR) |
This study |
| KH658 |
MS11 transformed with pKH209; mltG E213Q point mutant |
This study |
| KH673 |
KH658 transformed with pKH209 and pRS91; mltG and ltgA double point mutant |
This study |
| KH674 |
KH658 transformed with pKH209 and pRS92; mltG and ltgD double point mutant |
This study |
| RS555 |
MS11 transformed with pRS91; ltgA point mutant (E481A) (ErmR) |
Schaub et al. 2016 (28) |
| RS557 |
MS11 transformed with pRS92; ltgD point mutant (E158A) (ErmR) |
Schaub et al. 2016 (28) |
| |
|
|
|
Escherichia coli
|
|
|
| Plasmids for BACTH |
|
|
| pKT25 |
Cloning vector to add N-terminal T25 fragment for BACTH assays |
(Euromedex kit) |
| pUT18C |
Cloning vector to add N-terminal T18 fragment for BACTH assays |
(Euromedex kit) |
| pKT25-zip |
Positive control for BACTH assays |
(Euromedex kit) |
| pUT18C-zip |
Negative control for BACTH assays |
(Euromedex kit) |
