Table 2.
| Primer | Sequence | Plasmid or DNA |
|---|---|---|
| yceG start F Gib | GTGGCGGCCGCTCTAGAAGAGCGAACGGTATTGCGCTTC | pMRS1 |
| yceG start R Gib | GCATGGTTTACATGGTAGGATTCCCAGCATTC | pMRS1 |
| yceG stop F Gib | TCCTACCATGTAAACCATGCCGTCTGAAAAGTTTG | pMRS1 |
| yceG stop R Gib | CTGCAGCCCGGGGGATCCAGTCCGATACGCGCCATCGAT | pMRS1 |
| yceG pTEV5 F Nhe | AGAGCTAGCCCTAAAGACAACGGCAGGGC | pKH189, pKH193 |
| yceG pTEV5 R Xho | GGTACTCGAGGCTTGCAGTTCCGGTAAGGG | pKH189 |
| yceGFcomp Spe | TTTACTAGTCCTGTCCGGCGGGCAATTTG | pKH198 |
| new yceG R Hind | TGC AAG CTT CAT CCA AGC TGC GGT TAC TG | pKH193 |