Table 1.

Primer list for RT-PCR

Gene	Strand	Accession #	Sequence(5′-3′)	Siza	Exon	Source
Prox1	s	NM_008937	GTA AGA CAT CAC CGC GTG C	218	1	NIH Primer Tool
	as		TCA TGG TCA GGC ATC ACT GG		2
CD11b (Itgam)	s	NM_008401	ATG GAC GCT GAT GGC AAT ACC	203	13	MGH Primer Bank ID 668048a1
	as		TCC CCA TTC ACG TCT CCC A		14
Pdgfrα	s	NM_011058	AGA GTT ACA CGT TTG AGC TGT C	252	8	MGH Primer Bank 26349287a1
	as		GTC CCT CCA CGG TAC TCC T		10
						-
Myh11	s	NM_013607	AAG CTG CGG CTA GAG GTC A	238	33	MGH Primer Bank ID 7305295a1
	as		CCC TCC CTT TGA TGG CTG AG		34
CD117 (cKit)	s	NM_021099	CGC CTG CCG AAA TGT ATG ACG	162	21	(Drumm et al., 2018)
	as		GGT TCT CTG GGT TGG GGT TGC		23
Pdgfrβ	s	NM_008809	AGC TAC ATG GCC CCT TAT GA	367	16	(Basciani et al., 2004)
	as		GGA TCC CAA AAG ACC AGA CA		19
CD144 (VE-cadherin)	s	NM_009868	CTT CCT TAC TGC CCT CAT TGT	313	3	IDT Primer Quest
	as		CTG TTT CTC TCG GTC CAA GTT		5
Nos3 (eNOS)	s	NM_008713	ctg cca cct gat cct aac ttg	143	22	IDT Real time primer tool
	as		cag cca aac acc aaa gtc atg		23
Acta2 (Smooth Muscle Actin)	s	NM_007392	GAG CTA CGA ACT GCC TGA C	129	7	IDT TaqMan Mm.PT.58.16320644
	as		CTG TTA TAG GTG GTT TCG TGG A		8
CaV 1.2 exon1b	s	NM_001159533	ATG GTC AAT GAA AAC ACG AGG ATG		1	(Cheng et al., 2007)
	as		GGA ACT GAC GGT AGA GAT GGT TGC	234	2
CD34	as	NM_001111059	GGT ACA GGA GAA TGC AGG TC	119	2	IDT Mm.PT.58.8626728
	s	NM_133654	CGT GGT AGC AGA AGT CAA GT		1
Cspg4 (Ng2)	as	NM_139001	CTT CAC GAT CAC CAT CCT TCC	132	5	IDT Mm.PT.58.29461721
	s		CCC GAA TCA TTG TCT GTT CCC		6
-	-	-	-	-	-
Vimentin	s	NM_011701	CTG TAC GAG GAG GAG ATG CG	249	1	(Li et al., 2016)
	as		AAT TTC TTC CTG CAA GGA TT		3
Desmin	s	NM_010043	GTG GAT GCA GCC ACT CTA GC	218	3	MGH Primer Bank ID 33563250a1
	as		TTA GCC GCG ATG GTC TCA TAC		4
CD146 (Mcam)	s	NM_023061	CCC AAA CTG GTG TGC GTC TT	220	1	MGH Primer Bank 10566955a1
	as		GGA AAA TCA GTA TCT GCC TCT CC		3
KLF4	s	NM_010637	ATT AAT GAG GCA GCC ACC TG	400	1	(Majesky et al., 2017)
	as		GGA AGA CGA GGA TGA AGC TG		3
Ly6a (Sca1)	s	NM_001271416	CTC TGA GGA TGG ACA CTT CT	400	2	(Majesky et al., 2017)
	as		GGT CTG CAG GAG GAC TGA GC		4
Gli1	s	NM_01029	ATC ACC TGT TGG GGA TGC TGG AT	316	8	(Kramann et al., 2015)
	as		CGT GAA TAG GAC TTC CGA CAG		10
CD29 (Itgb1)	s	NM_010578	TCG ATC CTG TGA CCC ATT GC	170	14	NIH Primer Tool
	as		AAC AAT TCC AGC AAC CAC GC		15
CD105 (Endoglin)	s	NM_007932	TGA GCG TGT CTC CAT TGA CC	416	11	NIH Primer Tool
	as		GGG GCC ACG TGT GTG AGA A		15
CD44	as	NM_009851	CAC CAT TTC CTG AGA CTT GCT	148	19	IDT Mm.PT.58.12084136
	s		TCT GAT TCT TGC CGT CTG C		18
CD31 (Pecam1)	s	NM_008816	CTG CCA GTC CGA AAA TGG AAC	218	7	MGH Primer Bank ID 6679273a1
	as		CTT CAT CCA CTG GGG CTA TC		8
GJC1 (Connexin 45)	s	NM_008122	GGT AAC AGG AGT TCT GGT GAA	140	2	IDT Mm.PT.58.8383900
	as		TCG AAA GAC AAT CAG CAC AGT		3
Anoctamin 1 (TMEM16A)	s	NM_178642	ggc att tgt cat tgt ctt cca g	141	25	IDT Real time primer tool
	as		tcc tca cgc ata aac agc tc		26
CD45 (Ptprc)	s	NM_001111316	ATG CAT CCA TCC TCG TCC AC	225	29	NIH Primer Tool
	as		TGA CTT GTC CAT TCT GGG CG		31
MGH Harvard Primer Bank (Wang and Seed, 2003; Spandidos et al., 2008; Spandidos et al., 2010)