Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (human) | HEK293 | ATCC | CRL-1573 | |
| Cell line (human) | HEK293T | ATCC | CRL-3216 | |
| Cell line (human) | HEK293T tauRD P301S v2L FRET Biosensor | Produced by Diamond Lab |
Stably expresses mClover3 and mCerulean3 tagged tauRD monomers. |
|
| Cell line (human) | HEK293 DS1 tauRD(P301L-V337M)-YFP | Produced by Diamond Lab | Stably propagates diffuse tauRD monomers. | |
| Cell line (human) | HEK293 DS10 tauRD(P301L-V337M)-YFP | Produced by Diamond Lab | Stably propagates a unique tau strain. | |
| Cell line (human) | HEK293 OFF1::DS10 tauRD(P301L-V337M)-YFP | Produced by Diamond Lab | Stably propagates a unique tau strain under a tetracycline-repressible promoter. |
|
| Biological sample (human) | Alzheimer’s Disease subject brain | UT Southwestern Alzheimer’s Disease Center | ||
| Biological sample (human) | Progressive Supranuclear Palsy subject brain | UT Southwestern Alzheimer’s Disease Center | ||
| Biological sample (human) | Corticobasal Degeneration subject brain | UT Southwestern Alzheimer’s Disease Center | ||
| Antibody | Rabbit polyclonal anti-DnaJC7 | Proteintech | 11090-1-AP | 1:2000 dilution |
| Antibody | Rabbit polyclonal anti-DnaJB6 | Proteintech | 11707-1-AP | 1:2000 dilution |
| Antibody | Rabbit polyclonal anti-Beta-Tubulin | Proteintech | 10094-1-AP | 1:5000 dilution |
| Antibody | Donkey-anti-rabbit HRP- linked F(ab’)2 | Cytiva | NA9340-1ML | 1:5000 dilution (DnaJC7, Beta-Tubulin), 1:4000 dilution (DnaJB6) |
| Antibody | Mouse monoclonal anti-GAPDH | Proteintech | 60004-1-Ig | 1:10000 |
| Antibody | Mouse monoclonal anti-Beta-Actin | Proteintech | 66009-1-Ig | 1:5000 dilution |
| Antibody | Goat-anti-mouse H&L (HRP) | Abcam | ab6789 | 1:10000 dilution (GAPDH), 1:5000 dilution (Beta-Actin) |
| Peptide, recombinant protein | Human tau 2N4R (Full Length WT-tau) fibrils | Produced by Diamond Lab | MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKESPLQ TPTEDGSEEPGSETSDAKSTPTAEDVTAPLVDEGAPGKQAAAQPHTEIPEGTT AEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATP RGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTP GSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRL |
|
| Recombinant DNA reagent | gRNA-resistant WT DnaJC7 cDNA sequence | gBlock from IDT, cloned by VAP | The sequence can be found in Materials and methods: Design of a DnaJC7 construct resistant to targeting by used gRNA sequences |