Table 2.
Reverse transcription-quantitative polymerase chain reaction primers
|
Primer
|
Sequence (5’-3’)
|
| miR-151-3p-F | ACACTCCAGCTGGGCUAGACUGAGGCUCC |
| miR-151-3p-R | CTCAACTGGTGTCGTGGA |
| miR-19b-3p-F | ACACTCCAGCTGGGUGUGCAAAUCCAUGCAA |
| miR-19b-3p-R | CTCAACTGGTGTCGTGGA |
| miR-106b-5p-F | ACACTCCAGCTGGGUAAAGUGCUGACAGU |
| miR-106b-5p-R | CTCAACTGGTGTCGTGGA |
| miR-30e-5p-F | ACACTCCAGCTGGGUGUAAACAUCCUUGAC |
| miR-30e-5p-R | CTCAACTGGTGTCGTGGA |
| miR-122-5p-F | ACACTCCAGCTGGGUGGAGUGUGACAAUGG |
| miR-122-5p-R | CTCAACTGGTGTCGTGGA |
| miR-29a-3p-F | ACACTCCAGCTGGGUAGCACCAUCUGAAAU |
| miR-29a-3p-R | CTCAACTGGTGTCGTGGA |
| miR-29c-3p-F | ACACTCCAGCTGGGUAGCACCAUUUGAAAU |
| miR-29c-3p-R | CTCAACTGGTGTCGTGGA |
| miR-497-5p-F | ACACTCCAGCTGGGCAGCAGCACACUGUGG |
| miR-497-5p-R | CTCAACTGGTGTCGTGGA |
| miR-101a-3p-F | ACACTCCAGCTGGGUACAGUACUGUGAUA |
| miR-101a-3p-R | CTCAACTGGTGTCGTGGA |
| miR-741-3p-F | ACACTCCAGCTGGGAAAGAUGCCACGCUAU |
| miR-741-3p-R | CTCAACTGGTGTCGTGGA |
| miR-503-F | ACACTCCAGCTGGGTAGCAGCGGGAACAGTA |
| miR-503-R | CTCAACTGGTGTCGTGGA |
| miR-671-F | ACACTCCAGCTGGGUCCGGUUCUCAGGGC |
| miR-671-R | CTCAACTGGTGTCGTGGA |
| U6-F | CTCGCTTCGGCAGCACA |
| U6-R | AACGCTTCACGAATTTGCGT |
| SIRT4-F | TCCTGGGAGTGGACAGAATGA |
| SIRT4-R | CTGTGGATCCATGGGAACGC |
| Caspase 12-F | TGCCAATTCCGACAAACAGC |
| Caspase 12-R | CTGGATTCCCTGAGGAACTGT |
| GRP78-F | AACCCAGATGAGGCTGTAGCA |
| GRP78-R | ACATCAAGCAGAACCAGGTCAC |
| CHOP-F | CCAGCAGAGGTCACAAGCAC |
| CHOP-R | CGCACTGACCACTCTGTTTC |
| β-actin-F | CACCCGCGAGTACAACCTTC |
| β-actin-R | CCCATACCCACCATCACACC |
F: Forward; R: Reverse; miRNA/miR: microRNA; SIRT4: Sirtuin 4; CHOP: C/EBP-homologous protein; GRP78: Glucose regulated protein 78.