Table 2.
Enzyme | POX | LOX | DLD | ALDH | GDH | |||||
---|---|---|---|---|---|---|---|---|---|---|
PLL | 0 mM | 1 mM | 0 mM | 1 mM | 0 mM | 1 mM | 0 mM | 1 mM | 0 mM | 1 mM |
KM (mM) | 0.58 ± 0.06 | 0.82 ± 0.07 | 0.98 ± 0.23 | 0.14 ± 0.02 | 0.66 ± 0.15 | 0.93 ± 0.13 | 0.53 ± 0.09 | 0.40 ± 0.08 | 0.36 ± 0.03 | 0.31 ± 0.05 |
kcat (s-1) | 1.81 ± 0.06 | 3.81 ± 0.11 | 2.38 ± 0.16 | 8.17 ± 0.16 | 1.1 ± 0.03 | 3.47 ± 0.06 | 3.31 ± 0.13 | 14.1 ± 0.06 | 4.54 ± 0.07 | 6.10 ± 0.19 |
kcat/KM | 3.12 | 4.65 | 2.43 | 58.4 | 1.67 | 3.72 | 6.25 | 35.25 | 12.61 | 19.68 |
PLL, Poly-l-lysine; POX, Pyruvate oxidase; LOX, l-Lactate oxidase; DLD, d-Lactate dehydrogenase; ALDH, Aldehyde dehydrogenase; GDH, Glucose dehydrogenase.