Fig. 1. Strategies to increase cord blood units availability for transplantation.

a Scatter plot of CB units cryopreserved in the Héma-Québec CB bank. Black dots represent individual CB units according to total nucleated cells (TNC) and CD34⁺ cell dose adjusted for a 70 kg patient. Blue square, percentage of CB units meeting minimal cell dose criteria for single CB transplantation; red square, percentage of CB units meeting minimal cell dose criteria for UM171-expanded single CB transplantation. The remaining 55% of CB units do not meet minimum criteria for therapeutic use; thus, improved expansion technologies would allow the use of these smaller CBs and likely improve the HLA match of the CBs selected for transplantation as number of available CBs would increase. b Representation of the molecular pathways activated by the chemical components of the 3a medium (740Y-P, butyzamide and UM171) described by Sakurai et al.¹⁴.