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. 2023 Sep 1;13:14360. doi: 10.1038/s41598-023-40751-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Anchorage-dependent adjuvant therapy assay in Ewing sarcoma cells. (A) Schematic design of assay. (B) Adjuvant assay results in cell lines A673 and SK-N-MC (type 1 EWS) as well as SK-ES-1 and RDES (type 2 EWS). The cells were treated with adjuvant drug candidates for 21 days after 3-day etoposide treatment. The surviving cells were measured using CTG. Statistical analysis was performed using two sided t test. ***p < 0.001; **p < 0.01; *p <0.05 versus chemo-only. Chemo, chemotherapy (etoposide). All experiments included at least three replicates.