Fig. 6. Transfer of cecal microbiota from mice fed diet A protects against obesity, impairment of glucose metabolism and steatosis in mice fed the MF diet.
Mice were inoculated with cecal microbiota from mice fed MF or A diet and subsequently fed MF diet for 9 weeks. a Body weight, b eosin-hematoxylin staining of liver tissue (scale bar = 100 µm), c steatosis score, d liver triglyceride concentration, e hepatic expression of Hmgcr determined by qPCR, f fecal free fatty acids, and g ratio between saturated and unsaturated fecal free fatty acids. a–e: n = 15 (MF microbiota), 14 (A microbiota); f, g: n = 10 (MF microbiota) and 9 (A microbiota). Significant p values determined by two-sided Mann–Whitney U-test are displayed in the figure. Data are presented as mean ± SEM. Hmgcs1 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1. See also Supplementary Figs. 7 and 8. Source data are provided as a Source data file.