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N-ribosidase activity of TvRH at 37 °C and pH 6.5
| Substrate | kcat (s−1) | Km (μM) | kcat/Km (s−1 M−1) |
|---|---|---|---|
| Nicotinamide riboside | 10.9 ± 0.24 | 99.1 ± 8.2 | 1.1·105 |
| Uridine | 2.0 ± 0.08 | 58.6 ± 7.1 | 0.34·105 |
The steady-state kinetic parameters were obtained from the direct fit of initial velocities to the Michaelis–Menten equation using GraphPad Prism. Errors reported are standard errors from the fit.
