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Representative images of neutrophil elastase (green) and caspase-3 (red) immunostaining at 1 and 5 hours after seeding onto control, human acellular vessel (HAV), expanded polytetrafluorethylene (ePTFE), and control with phorbol-12-myristate-13-acetate (PMA) (100 ng/mL). Nuclei (blue) counterstained with 4′,6-diamidino-2-phenylindole (DAPI). Scale bars, 50 μm. The white arrowheads in ePTFE samples show the leaking DNA or neutrophil elastase.
