Fig. 10.

(1) MG-63 cells on Ti–6Al–4V (a, b) and iron (c, d) scaffolds; live cells, green; dead cells, red. Scale bars indicate 300 mm (a, c) and 30 mm (b, d) (adapted from Ref. [40]). (2) Preosteoblasts after 3 days of culture in (a) 100%, (b) 75%, and (c) 50% iron extracts, (a–c) rhodamine phalloidin (red) and DAPI (blue) stained; morphology of the cells after 3 days of direct cell culture on the iron scaffolds (d-f) calcein acetoxymethyl (green, indicating living cells) and ethidium homodimer-1 (red, indicating dead cells) (adapted from Ref. [46]). (3) Corrosion of iron stent produces ROS by the Fenton reaction that can induce a cascade of oxidations on cell components.