Fig. 2.

The number of P. aeruginosa and S. aureus CFU recovered after single-dose treatment of 24 h old dual-species biofilms. EPA1+SAFA: phage EPA1 and SAFA were applied simultaneously at MOI of 1. Sequential means that phage EPA1 and SAFA were applied simultaneously at MOI of 1; subsequently GEN was applied (4 μg/mL, i.e. the MIC for P. aeruginosa) with a 6 h delay. (^) Statistical differences between the control and treated biofilms. (#) Statistical differences between the compared treatment groups. Statistical differences were determined by two-way repeated-measures analysis of variance (ANOVA) with Tukey’s multiple comparison tests (p < 0.001). Values are the average of three technical repeats in duplicate, error bars indicate standard deviation.