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. 2023 Sep 2;11(3):294–308. doi: 10.2478/jtim-2023-0105

Figure 7.

Figure 7

FOSL2 upregulates SGK1 expression by enhancing the activity of SGK1 transcription. (A, B) The qRT-PCR and western blotting analysis of SGK1 in HK-2 cells after FOSL2 silencing or overexpression. (C) Schematic diagram of four potential FOSL2-binding sites and truncated fragments on the SGK1 promoter. (D) The regulation of FOSL2 on SGK1 transcription activity was evaluated by dual-luciferase assay. (E) A dual-luciferase assay was performed to detect the binding site of FOSL2 on SGK1 promoters. The values are expressed as mean ± standard deviation. **P < 0.01 versus siNC, vector, or control group; ##P < 0.01 versus TGF-β1 + siNC. qRT-PCR: quantitative real-time polymerase chain reaction; TGF-β1: transforming growth factor-β1; SGK1: serum/glucocorticoid regulated kinase 1; FOSL2: fos-related antigen 2.