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Effects of TP on cell apoptosis in the TM3 cells. A–D. Apoptosis in TM3 cells was detected after 24 h of treatment with TP at various concentrations (B: 50 nmol/L; C:100 nmol/L; D: 200 nmol/L) or a control (A : 0.1% DMSO) using Annexin V-FITC/PI binding and measured using flow cytometry analysis. E: The chart illustrates apoptosis proportion from three independent experiments (**P < 0.05 vs. control group, n = 3). TP: triptolide; DMSO: dimethyl sulfoxide.
