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. 2023 Sep 2;14:5355. doi: 10.1038/s41467-023-41121-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a illustration of the mechanism of GSDME-dependent pyroptosis induced by COF-818 and COF-919. Quantitative evaluation of the representative pyroptosis factors, ATP (b) and LDH (c) in 4T1 cells after various treatments; data are presented as mean ± SEM (n = 3 independent samples). d Representative images of HMGB1 immunofluorescence staining in 4T1 cells after treatment with different samples. e Evaluate the expression levels of cleaved Caspase-3 and GSDME-N in 4T1 cells through western blot analysis; Western blot was done thrice independently with similar results. f Representative images of 4T1 cell morphology change after various treatments; the photographs of cell morphology were representative of those generated from three independent samples, each group with similar results; scale bar = 50 μm. Statistical significance was calculated via one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.