Skip to main content
. 2023 Sep 2;14(9):585. doi: 10.1038/s41419-023-06112-4

Fig. 4. BCL2L13 knockdown inhibits mitophagy in GBM cells in vitro.

Fig. 4

A–C TEM images of sh-NC/BCL2L13 GBM#P3 and GBM#BG5 cells. Arrows highlight autophagosomes (arrows). The scale bar in the original image represents 0.8 μm, and the scale bar in the enlarged image represents 0.3 μm. D Western blotting analysis to detect protein levels of LC3B, BECN1, and GAPDH (control for loading) in sh-NC/BCL2L13 GBM#P3 and GBM#BG5 cells. Data are representative of three independent experiments. E Confocal microscopy images of sh-NC/BCL2L13 GBM#P3 and GBM#BG5 cells after co-expressing RFP-mito and GFP-LC3B (scale bars: 10 μm). F Representative confocal images are of GBM#P3 and GBM#BG5 cells expressing mt-Keima transfected with sh-NC or three shRNAs targeting BCL2L13 (sh-BCL2L13#2, and #3). G Western blotting analysis to detect protein levels of NDP52, PINK1, Parkin, Optineurin, BNIP3/NIX, BNIP3, and GAPDH (control for loading) in sh-NC/BCL2L13 GBM#P3 and GBM#BG5 cells. All data are expressed as the mean ± SD of values from experiments performed in triplicate.