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. 2023 Sep 2;14(9):585. doi: 10.1038/s41419-023-06112-4

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — A Confocal microscopy analysis of mitochondrial morphology was visualized with MitoTracker Red in A172-EV/BCL2L13/BCL2L13-MUT cells. B TEM images of A172-EV/BCL2L13/BCL2L13-MUT cells. Arrows highlight autophagosomes (arrows). The scale bar in the original image represents 0.8 μm, and the scale bar in the enlarged image represents 0.3 μm. C Representative confocal images are of A172 cells expressing mt-Keima transfected with EV/BCL2L13/BCL2L13-MUT. D Western blotting analysis to detect protein levels of NDP52, PINK1, Parkin, Optineurin, BNIP3/NIX, BNIP3, LC3B and GAPDH (control for loading) in A172 cells transfected with EV/BCL2L13/BCL2L13-MUT. E Confocal microscopy images of GFP-LC3B and RFP-mito expressing A172-EV/BCL2L13 cells after Mdivi-1 (50 µM) treatment for 24 h (scale bars: 10 μm). F Western blotting analysis for LC3B in A172-EV/BCL2L13 cells after Mdivi-1 treatment. G Representative images and statistic results of migration and invasion ability of A172-EV/BCL2L13 cells treated with mdivi-1. All data are expressed as the mean ± SD of values from experiments performed in triplicate. ***P < 0.001 compared between the two treatments indicated.