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. 2023 Aug 21;14(13):2574–2584. doi: 10.7150/jca.86940

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circROBO1 is upregulated in prostate cancer cells and tissues. (A) Comparison of the expression level of circROBO1 in normal WPMY1 cells and prostate cancer cells. (B) The expression level of the circROBO1 expression level in ten pairs of prostate cancer tissues and adjacent normal tissues, detected by qPCR analysis. (C) RNase R assays were used to examine circROBO1's circular feature in C4-2B and 22RV1 prostate cancer cell line. (D) Actinomycin D treated assays showed that circular ROBO1 transcripts were more stable than linear ROBO1 transcripts in C4-2B and 22RV1 prostate cancer cell line.