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. 2023 Aug 21;14(13):2574–2584. doi: 10.7150/jca.86940

Figure 4.

Figure 4

Glycolysis regulating enzyme PGK1 is the downstream target of miR-556-5p in prostate cancer. (A) The downstream target of miR-556-5p, PGK1 mRNA, was predicted by TargetScan online. (B) Comparison of the expression level of PGK1 in normal WPMY1 cells and prostate cancer cells. (C) The expression level of the PGK1 expression level in ten pairs of prostate cancer tissues and adjacent normal tissues, detected by qPCR analysis. (D-E) Enrichment of circROBO1, PGK1 and miR-556-5p on AGO2 in C4-2B and 22RV1 prostate cancer cell lines assessed by RIP assay. (F-G) Dual luciferase reporter assay of C4-2B and 22RV1 prostate cancer cell lines transfected with miR-556-5p mimics and PGK1 mRNA 3'-UTR wild-type or mutant-type luciferase vectors. (F) Enrichment of PGK1 mRNA to AGO2 was significantly increased after the suppression of circROBO1 in C4-2B and 22RV1 prostate cancer cell lines.