Figure 3.

AKT-mediated EZH2 phosphorylation induces EZH2’s interaction with β-catenin

(A) Western blots of EZH2 immunoprecipitations (IP) performed using nuclear lysates prepared from SW480 cells untreated or treated with 250 μM H₂O₂ for 30 min. IgG IP serves as a negative control. Input is nuclear lysates used for IP.

(B) EZH2 IP performed using nuclear lysates prepared from human colorectal cancer organoids treated as in A.

(C and D) β-catenin IP performed using nuclear lysates prepared from empty vector (EV) and PTEN knockdown (KD) RKO (C) and Caco2 (D) cells.

(E) β-catenin IP performed using nuclear lysates prepared from EZH2 KD and EV SW480 cells treated as in A.

(F) β-catenin IP performed using nuclear lysates prepared from PTEN KD and EV SW480 cells treated with 2 μM EZH2 inhibitor (EZH2i, GSK-503) or DMSO for 72 h.

(G) HA IP performed using nuclear lysates prepared from HA-EZH2 wildtype (WT) and HA-EZH2 phospho-null (PN) S21A expressing SW480 cells treated as in A. See also Figure S3.