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. 2023 Aug 16;26(9):107630. doi: 10.1016/j.isci.2023.107630

Figure 4.

Figure 4

EZH2 mediates β-catenin trimethylation at K49

(A) Trimethyl lysine (Me3-K) immunoprecipitation (IP) performed using nuclear lysates prepared from Flag β-catenin WT and Flag β-catenin K49R expressing SW480 cells treated with 250 μM H2O2 for 30 min and Flag β-catenin WT expressing untreated cells. Input is nuclear lysates used for IP. IP with IgG serves as a negative control.

(B) Trimethyl lysine (Me3-K) IP performed using nuclear lysates prepared from Flag β-catenin WT and Flag β-catenin K49R expressing PTEN knockdown (KD) and empty vector (EV) SW480 cells. IP with beads only serves as a negative control.

(C) β-catenin IP preformed using nuclear lysates prepared from EV or PTEN KD SW480 cells treated with 2 μM EZH2 inhibitor (EZH2i, GSK-503) or DMSO for 72 h. See also Figure S4.