Figure 2.

Characterization of the global proteome

(A) Approximately 6,000 proteins were identified from otic tissues across all analyzed developmental stages and the five biological replicates. Proteins correlated with publicly available proteomic and transcriptomic datasets from inner ear tissues. A number of proteins previously reported in inner ear tissues (red) and hair cells (blue) were included in our dataset. Additional proteins correlated with deafness genes reported on OMIM (green).

(B) Over-representation analysis (PantherDB) for biological processes demonstrating a fold enrichment >5 indicated an enrichment of processes such as mRNA processing and splicing in the otic proteome.

(C) Over-representation analysis (PantherDB) for pathways demonstrated an enrichment in pathways such as Integrin, Wnt, and FGF signaling.