REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-Digoxigenin-AP, Fab fragments	Millipore Sigma	Cat#11093274910
Chemicals, peptides, and recombinant proteins
Human Chorionic gonadotropin	Sigma	Cat#CG10
C18 EASY-Spray HPLC column	Thermo	Cat#03-052-574
TPCK-modified trypsin	Thermo	Cat#20233
Pierce BCA Protein Assay kit	Thermo	Cat#23227
Pierce Quantitative Colorimetric Peptide Assay kit	Thermo	Cat#PI23275
Pierce High pH reverse phase peptide fractionation kit	Thermo	Cat#84868
Deposited data
RNAseq data	NCBI	Submission ID: SUB13273105; BioProject ID: PRJNA967984
Mass spectrometry proteomics data	ProteomeXchange Consortium via the PRIDE partner repository	PXD041991; https://doi.org/10.6019/PXD041991
Experimental models: Organisms/strains
129/S6 Six1-deleted mouse line	Dr. Kiyooshi Kawakami, Jichi Medical School	Ozaki et al., 200465
Xenopus laevis, wild type	Nasco Education, Ft. Atkinson, WI, USA	No longer available
Oligonucleotides
5′ AATTCATTTTTGGAGCTGTACTAAG 3′	Gene-tools	custom
5′ TCACAGCAGCTAAGAGAGAAAGCCA 3′	Gene-tools	custom
Recombinant DNA
Xenopus laevis dlx5 cDNA	Dr. Thomas Sargent, NIH-NICHD	Luo et al., 200166
Xenopus laevis pax2 cDNA	Dr. Andre Brandli, Ludwig-Maximilians Univ Munchen	Heller and Brandli 199767
Human TGFBI	Origene	Cat#RC200411
Software and algorithms
Skyline	SCIEX	https://sciex.com/products/software/skyline-software
Proteome Discovery V2.1	ThermoFisher Scientific	Cat#OPTON-31103 for v3.0
ComplexHeatmap package, 4.2	In R
PantherDB	Panther Classification System	https://pantherdb.org/
OriginPro	OriginLab	https://www.originlab.com/
STAR Aligner v2.5.2b	https://www.encodeproject.org/software/star/	http://code.google.com/p/rna-star/
featureCounts, subread package v.1.5.2	Bioconductor Rsubread	https://subread.sourceforge.net/featureCounts.html
cellSense Entry	Olympus	https://www.olympus-lifescience.com/en/software/cellsens/
Prism 9.4.0	GraphPad	https://www.graphpad.com/features
String	String database	https://string-db.org/
Xenopus laevis proteome	UniProt58	https://www.uniprot.org/
PHROG database	Peshkin et al., 201514	http://kirschner.med.harvard.edu/MADX.html
ThorImageOCT v.5.1.1	Thorlabs	https://www.thorlabs.com/newgrouppage9.cfm?objectgroup_id=7982