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. 2023 Jun 25;114(9):3636–3648. doi: 10.1111/cas.15882

FIGURE 4.

FIGURE 4

Induction of LRIG1 by bone morphogenetic protein (BMP) inhibition is involved in the growth suppression of colorectal cancer (CRC) organoids. (A) Scatter plot showing the correlation of growth and LRIG1 induction in the presence of 0.1 μM LDN193189 (LDN) relative to LDN‐free condition. Each dot represents an individual CRC organoid line (n = 18). r, Spearman's rank correlation coefficient; p = 0.000195, Spearman's rank correlation test. (B) Immunoblotting analysis of C45 organoid with inducible shLRIG1 cultured for 48 h with LDN and at the indicated concentration (nM) in the presence (+) or absence (−) of doxycycline (DOX). Proteins were detected with indicated Abs. (C) Cell viability assay results for C45 and CB3 organoids with inducible shLRIG1. Organoids were treated with 0.1 μM LDN in the presence (+) or absence (−) of DOX for 5 days. Data are presented as mean + SD. n = 5. *p < 0.05, t‐test with Bonferroni correction. (D) Representative bright‐field images of C45 organoids with inducible shLRIG1. Organoids were treated with 0.1 μM LDN in the presence (+) or absence (−) of DOX for 5 days. Scale bar, 100 μm. ACTB, β‐actin; Ctrl, control; EGFR, epidermal growth factor receptor; NS, not significant.