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. 2023 Jul 13;114(9):3759–3769. doi: 10.1111/cas.15903

FIGURE 1.

FIGURE 1

Viral replication of the interferon (IFN)‐expressing oncolytic adenovirus (IFN‐OAd) combined with chemotherapy. (A) Structure of the IFN alpha‐expressing oncolytic adenovirus (IFN‐OAd). IFN‐OAd is a wild‐type replication oncolytic adenovirus expressing the hamster IFN alpha gene from the adenoviral E3 region. (B–E) Viral replication of IFN‐OAd with gemcitabine (GEM) and paclitaxel (PTX) in vitro. HP‐1 cells were infected with IFN‐OAd. Twenty‐four hours after infection, PBS (Without chemo), GEM (GEM only: 2.5 or 10 nM GEM), PTX (PTX only: 2.5 nM or 10 nM PTX), and GEM+PTX (2.5 nM GEM + 2.5 nM PTX or 10 nM GEM + 10 nM PTX) were added to each well. The viral copy number was measured by quantitative PCR (qPCR) on Day 7. The results are shown as E4 copy number per dish and ratio compared to without chemo (n = 3). Data are presented as mean ± standard deviation (n = 3, *p < 0.05). ADP, adenoviral death protein; Ham‐IFN, hamster interferon alpha; L‐ITR, left inverted terminal repeat; RGD, RGD‐4C (arginine‐glycine‐aspartic) motif; R‐ITR, right inverted terminal repeat.