FIGURE 3.

Antitumor effect of interferon (IFN)‐expressing oncolytic adenovirus (IFN‐OAd) combination therapy in hamster syngeneic subcutaneous tumor model. (A) Treatment schedule fot hamsters. HP‐1 cells were subcutaneously inoculated. Once the average tumor volume reached 200 mm³, the hamsters were divided into four groups: Control (n = 4), chemotherapy (gemcitabine [GEM] + nab‐paclitaxel [nab‐PTX]) + radiation (CR) (n = 10), IFN‐OAd (V) (n = 6), and CRV (n = 5) groups. A single dose of virus or vehicle alone (PBS) was injected into the tumors on Day 0. Radiation was performed on Day 3. Intraperitoneal injection of 20 mg/kg GEM + 2 mg/kg nab‐PTX was given twice weekly. (B) The average body weight (g) of each group on Day 21. Data are presented as mean ± standard deviation. (C) Average tumor volume of CRV. The average tumor volumes on Day 21 of the control, CR, V, and CRV were 2027 ± 1296, 908 ± 849, 767 ± 593, and 202 ± 161 mm³, respectively. (D) Individual relative tumor volume from baseline (Day 0). The dashed line represents the baseline (=1). (E–H) Individual tumor volume of control, CR, V, and CRV. (n.s., not significant; *p < 0.05).