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. 2023 Jul 12;114(9):3666–3678. doi: 10.1111/cas.15901

FIGURE 2.

FIGURE 2

Effects of Fusobacterium nucleatum on migration and invasion of pancreatic cancer cells. (A) Representative photomicrographs of migrating and invading SUIT‐2 cells cocultured with F. nucleatum and P. gingivalis or monocultured. (B) F. nucleatum promoted the migration and invasion of SUIT‐2 cells compared with P. gingivalis‐cocultured and monocultured cells. (C) Proliferation rate of SUIT‐2 cells cocultured with F. nucleatum was similar to that of P. gingivalis‐cocultured and monocultured SUIT‐2 cells. (D) Representative human cytokine array images of conditioned medium derived from SUIT‐2 cells cocultured with F. nucleatum and P. gingivalis or monocultured. Black boxes: positive controls; orange boxes: CXCL1; green boxes: IL‐8. CXCL1 and IL‐8 were upregulated in the conditioned medium of SUIT‐2 cells cocultured with F. nucleatum, but not with P. gingivalis or monocultured. (E) CXCL1 (left) and IL‐8 (right) expression was increased in SUIT‐2 cells cocultured with F. nucleatum, but not with P. gingivalis. (F) Representative photomicrographs of migrating F. nucleatum‐cocultured SUIT‐2 cells with or without the CXCR2 inhibitor (SB225002). The CXCR2 inhibitor significantly suppressed migration of F. nucleatum‐cocultured SUIT‐2 cells. Original magnification: ×40; scale bar 100 μm. *p < 0.05, **p < 0.01.