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. 2023 Aug 22;3(8):100566. doi: 10.1016/j.crmeth.2023.100566

Figure 1.

Figure 1

Binding profiles of mAbs generated using the influenza antigen microarray (AM)

Binding profiles of 23 influenza mAbs to a panel of 43 influenza recombinant HA proteins from influenza A/H1N1, A/H3N2, B, A/H5N1, A/H7N9, and A/H9N2 subtypes. Each mAb was profiled in multiple dilutions ranging from 6 to 0.375 μg/mL. For each mAb antigen pair, the area under the curve (AUC) is presented, with higher AUC values in darker colors. AUC values were calculated from the median fluorescence intensities (MFI) across all dilutions. AUC values that were smaller than 100 were considered background (light gray). The top bars represent the binding site and the binding profile of each mAb as previously characterized (see Table S1). mAbs are colored by epitope specificities as described in the legend. mAbs colored in dark green, light green, and brown bind the HA stalk, and those colored in dark blue and light blue bind the HA head. mAbs were clustered based on their binding profiles as represented by the dendrogram on the top of the figure. Four major clusters were identified that overall separate mAbs by their binding sites, other than the central stalk antibodies, which separate into two distinct clusters separated by binding breadth.