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. 2023 Aug 31;11(8):e007253. doi: 10.1136/jitc-2023-007253

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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VBL-induced ROS repolarizes TAMs. (A, B) Flow cytometry analysis of cytosolic ROS (A) and mitoROS (B) levels in macrophages sorted from Vehicle/VBL treated MC38 tumors. n=6. (C) Flow cytometry analysis for percentages of M1 (F4/80⁺CD11b⁺CD86⁺) and M2 (F4/80⁺CD11b⁺CD206⁺) in BMDMs under the corresponding treatment for 24 hours. n=6. (D) T cell proliferation ratio after co-culture with macrophages by direct contact or transwell. n=4–5. (E, F) Flow cytometry analysis of cytosolic ROS (E, n=4) and mitoROS (F, n=5) levels in BMDMs with or without Cyba knockdown. Data are presented as mean±SD. P values were determined by a two-tailed Student’s t-test. *p<0.05, **p<0.01, ***p<0.001. BMDMs, bone marrow-derived macrophages; MFI, mean fluorescence intensity; ROS, reactive oxygen species; VBL, vinblastine.