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. 2023 Aug 31;11(8):e007253. doi: 10.1136/jitc-2023-007253

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See http://creativecommons.org/licenses/by-nc/4.0/.

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ROS-activated TFEB promotes lysosome activation and biogenesis. (A) RNA-sequencing data was collected from BMDMs (Mϕ in vitro) with or without VBL treatment for 24 hours, and the heat map was created based on normalized FPKM of genes in the lysosome pathway. (B) Immunostaining with F4/80 and LysoTracker showed the changes in lysosome levels in BMDMs after VBL treatment. (C) Statistical results of LysoTracker MFI in panel (B). n=9 (control). n=6 (VBL). (D) Representative flow cytometry results of Lamp1 in BMDMs after VBL treatment for 24 hours. (E) Statistical results of Lamp1 MFI in panel (D). n=4. (F) Immunostaining with TFEB, F4/80, and Hoechst in BMDMs after indicated treatment for 24 hours. (G) Statistical results of TFEB and Hoechst colocalization in (F). n=12. Data are presented as mean±SD. P values were determined by a two-tailed Student’s t-test. *p<0.05, **p<0.01, ***p<0.001. BMDMs, bone marrow-derived macrophages; MFI, mean fluorescence intensity; VBL, vinblastine.