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. 2023 Aug 31;11(8):e007253. doi: 10.1136/jitc-2023-007253

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© Author(s) (or their employer(s)) 2023. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

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VBL reprograms macrophages to be tumoricidal through increased phagocytic capacity. (A) Schematic experimental workflow for evaluating the phagocytic capacity of macrophages. (B) Representative flow cytometry results of BMDMs after VBL treatment and co-culture with CFSE⁺ MC38 tumor cells. (C) Statistical results of CFSE⁺ BMDMs in (B). n=6. (D) Representative flow cytometry results of BMDMs after different treatments and co-culture with CFSE⁺C38 tumor cells. (E) Statistical results of CFSE⁺ BMDMs in (D). n=6. (F) Representative flow cytometry results of VBL-induced BMDMs after ROS scavenging (NAC) or lysosome inhibition (HCQ) and co-culture with CFSE⁺ MC38 tumor cells. (G) Statistical results of CFSE⁺ BMDMs in (F). n=6. Data are presented as mean±SD. P values were determined by a two-tailed Student’s t-test. **p<0.01, ***p<0.001. BMDMs, bone marrow-derived macrophages; CFSE, carboxyfluorescein succinimidyl amino ester; HCQ, hydroxychloroquine; NAC, N-acetylcysteamine; ROS, reactive oxygen species; VBL, vinblastine.