Table 2. Precision (%CV) and Accuracy (%RE) Results for Quality Control Samples (n = 6) of CCK8 and ppCCK(21–44), Prepared in Organoid Secretion Buffer and Quantified against a Calibration Curve.
spiked concentration (pg/mL) | 25 | 50 | 100 | 400 |
---|---|---|---|---|
CCK8 | ||||
mean | 24.8 | 52.6 | 118.1 | 402.9 |
standard deviation | 3.2 | 6.3 | 12.9 | 2.2 |
%CV | 12.8 | 12.0 | 10.9 | 0.5 |
%RE | –0.7 | 5.2 | 18.1 | 0.7 |
ppCCK(21–44) | ||||
mean | 25.8 | 42.0 | 118.3 | 437.2 |
standard deviation | 0.9 | 2.7 | 12.8 | 16.0 |
%CV | 3.3 | 6.5 | 10.8 | 3.7 |
%RE | 3.3 | –16.1 | 18.3 | 9.3 |