Figure 7. Formation of stable centromeric nucleosome requires tight coordination of centromeric DNA, Cse4^CENP‐A with its chaperone Scm3^HJURP and CCAN kinetochore protein association.

Schematic of centromeric nucleosome formation highlighting the different pathways that could lead to either short‐lived or long‐lived residences Cse4^CENP‐A as measured in TIRFM resident lifetime assays.