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Osteopontin affected bone metabolism through Notch pathway. A) RANK-positive rates of RAW264.7 cells in sh-NC, sh-NC + LY3039478, sh-OPN and sh-OPN + LY3039478 groups were detected by flow cytometry. B) Osteocalcin-positive rates of SaOS-2 cells in the four groups. C) Alizarin Red S staining was performed on the four groups of SaOS-2 cells to evaluate the mineralization.
