Fig. 4.

Direct regulation of MCM2 expression by miR‐139‐3p, miR‐378a‐5p, and miR‐2110 in LUAD cells. This figure shows dual‐luciferase reporter assay results following transfection with (A) miR‐139‐3p, (B) miR‐378a‐5p, and (C) miR‐2110. The upper panels are putative miR‐139‐3p, miR‐378a‐5p, and miR‐2110 binding sites predicted within the 3′‐UTR of MCM2 by TargetScanHuman database analysis. After 24 h co‐transfection of miRNA and wild‐type or deletion‐type psiCHECK‐2 vector, dual‐luciferase reporter assay was conducted (lower panel). Wild‐type vector contains the miRNA binding site of MCM2 3′‐UTR sequence, and deletion‐type one does not. Normalized data were calculated as the Renilla/firefly luciferase activity ratio. Luminescence activity was measured in triplicate. The data are mean and SD. One‐way ANOVA and Tukey tests for post hoc analysis. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, n.s., not significant. RSEM, RNA‐seq by Expectation‐Maximization.