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. Author manuscript; available in PMC: 2023 Sep 4.
Published in final edited form as: Nat Protoc. 2020 Nov 9;15(12):3942–3970. doi: 10.1038/s41596-020-0396-3

Fig. 3. Red arrow flag for checking the laser alignment.

Fig. 3

a, Front view of the red arrow flag when aligned with the laser beam. Region colored in light blue represents the laser beam. The regions colored in orange, light gray, and blue are the polyimide-coated wall, naked wall (transparent window), and the liquid inside the capillary, respectively. b, Zoom-in of the region illuminated by the laser beam, with detailed dimensions of the laser spot and the capillary diameters. c, Schematic of the laser burn mark after a 50-shot laser sequence. Dimensions are in line with those in b.