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A sample nano-flow LC gradient for analyzing FPOP samples on a Thermo Scientific Q Exactive mass spectrometer
| Retention Time (min) | Flow Rate (μl·min−1) | % Phase A | % Phase B | |
|---|---|---|---|---|
| 1 | 0 | 0.40 | 97.5 | 2.5 |
| 2 | 10 | 0.40 | 97.5 | 2.5 |
| 3 | 40 | 0.40 | 82.5 | 17.5 |
| 4 | 62 | 0.40 | 50.0 | 50.0 |
| 5 | 67 | 0.40 | 20.0 | 80.0 |
| 6 | 75 | 0.40 | 20.0 | 80.0 |
| 7 | 80 | 0.40 | 97.5 | 2.5 |
| 8 | 90 | 0.40 | 97.5 | 2.5 |
