Figure 4.

Comparison of the detection efficiency of ddPCR, RT-PCR, and ELISA for HDV. (a) The Venn diagram shows the detection number of ddPCR, RT-PCR, and ELISA for HDV in 44 clinical confirmed patients including 30 HDV infection subjects. The purple area shows the number of HDV antibodies positive detected by ELISA; the red area shows the number of HDV RNA detected by the ddPCR; the blue area shows the number of HDV RNA detected by the RT-PCR. (b) Comparison of the detection values between the ddPCR and RT-PCR. The measured values of RT-PCR were plotted on the X axis, the vertical dashed line denote the LLoQ of RT-PCR; the measured values of ddPCR were plotted on the Y axis, the parallel dashed line denotes the LLoQ of ddPCR. ddPCR, digital droplet PCR; ELISA, enzyme-linked immunosorbent assay; HDV, hepatitis delta virus; LLoQ, lower limit of quantitation; RT-PCR, reverse-transcriptase PCR.