| Genetic reagent (Mus musculus) |
C57BL/6J |
Jackson Laboratory |
Cat#000664; RRID:IMSR_JAX:000664
|
|
| Genetic reagent (M. musculus) |
Tsc1tm1Djk/J |
Jackson Laboratory |
Cat#005680; RRID:IMSR_JAX:005680
|
|
| Genetic reagent (M. musculus) |
Camk2a-Cre |
Jackson Laboratory |
Cat#005359; RRID:IMSR_JAX:005359
|
|
| Genetic reagent (M. musculus) |
cTag-PABP |
PMID:28910620
|
MGI:6116824 |
MGI symbol: Pabpc1tm1.2Rbd |
| Cell line (M. musculus) |
Neuro-2a |
ATCC |
Cat#CCL-131; RRID:CVCL_0470
|
|
| Cell line (Homo sapiens) |
HEK293T/17 |
ATCC |
Cat#CRL-11268; RRID:CVCL_1926
|
|
| Cell line (H. sapiens) |
MDA-MB-231 |
ATCC |
Cat#HTB-26; RRID:CVCL_0062
|
|
| Cell line (H. sapiens) |
BE2C |
ATCC |
Cat#CRL-2268 |
|
| Cell line (H. sapiens) |
CKO HEK293T |
PMID:26858452
|
|
|
| Cell line (H. sapiens) |
shCPSF6-BE2C |
This paper |
|
Figure 5A
|
| Cell line (H. sapiens) |
shCPSF6-MDA-MB-231 |
This paper |
|
Figure 6—figure supplement 1F
|
| Antibody |
Anti-beta actin (mouse monoclonal) |
ProteinTech |
Cat#66009-1-Ig; RRID:AB_2687938
|
1:10000 |
| Antibody |
Anti-alpha tubulin (mouse monoclonal) |
Millipore |
Cat#CP06; RRID:AB_2617116
|
1:3000 |
| Antibody |
Anti-TRIM9 (rabbit polyclonal) |
ProteinTech |
Cat#10786-1-AP; RRID:AB_2209130
|
1:2000 |
| Antibody |
Anti-S6, clone 5G10 (rabbit monoclonal) |
Cell Signaling Technology |
Cat#2217; RRID:AB_331355
|
1:2000 |
| Antibody |
Anti-Phospho-S6 (Ser240/244) (rabbit monoclonal) |
Cell Signaling Technology |
Cat#5364; RRID:AB_10694233
|
1:2000 |
| Antibody |
Anti-CPSF6 (rabbit polyclonal) |
Bethyl Laboratories |
Cat#A301-356A; RRID:AB_937781
|
1:2000 |
| Antibody |
Anti-BRD4 (rabbit polyclonal) |
Bethyl Laboratories |
Cat#A301-985A; RRID:AB_1576498
|
1:10000 |
| Antibody |
Anti-BRD4 (rabbit monoclonal) |
Abcam |
Cat#ab128874; RRID:AB_11145462
|
1:3000 |
| Strain, strain background |
NEB 5-alpha |
New England Biolabs |
Cat#C2987 |
|
| Strain, strain background |
NEB Stable |
New England Biolabs |
Cat#C3040 |
|
| Commercial assay or kit |
ProtoScript II First Strand cDNA Synthesis Kit |
New England Biolabs |
Cat#E6560 |
|
| Commercial assay or kit |
Q5 Site-Directed Mutagenesis Kit |
New England Biolabs |
Cat#E0554 |
|
| Commercial assay or kit |
NEB PCR Cloning Kit |
New England Biolabs |
Cat#E1202 |
|
| Commercial assay or kit |
10% Bis-Tris NuPAGE gels |
Invitrogen |
Cat#NP0301BOX |
|
| Commercial assay or kit |
3~8% Tris-Acetate NuPAGE gels |
Invitrogen |
Cat#EA0375BOX |
|
| Commercial assay or kit |
DNase I |
Invitrogen |
Cat#18068015 |
|
| Commercial assay or kit |
Trizol reagent |
Invitrogen |
Cat#15596018 |
|
| Commercial assay or kit |
PerfeCTa SYBR Green SuperMix |
QuantaBio |
Cat#95054–500 |
|
| Chemical compound, drug |
DMSO |
Sigma |
Cat#D2650 |
|
| Chemical compound, drug |
Torin-1 |
Cayman Chemical |
Cat#10997 |
|
| Chemical compound, drug |
DharmaFECT1 |
Horizon Discovery |
Cat#T-2001-02 |
|
| Chemical compound, drug |
DharmaFECT2 |
Horizon Discovery |
Cat#T-2002-02 |
|
| Chemical compound, drug |
X-tremeGENE 9 |
Sigma |
Cat#XTG9-RO |
|
| Sequence-based reagent |
Supplementary file 4
|
This paper |
|
Primers used |
| Sequence-based reagent |
Supplementary file 5
|
This paper |
|
siRNAs used |
| Recombinant DNA reagent |
Supplementary file 6
|
Addgene or this paper |
|
Plasmids used or generated |
| Software, algorithm |
CIMS package |
PMID:24407355
|
|
|
| Software, algorithm |
Kallisto |
PMID:27043002
|
|
|
| Software, algorithm |
Prism 9.5 |
GraphPad Software |
https://www.graphpad.com/; RRID:SCR_002798
|
|
