Fig. 3.

Cytotoxicity of G-NK cells toward GPC3⁺ Tumor cells. A Apoptosis/necrosis of HepG2 cells incubated with NK, R-NK and G-NK cells for 1 h and washed twice to remove unbinding NK cells, and then further incubated for 24 h at the E/T ratio of 10:1. B–D Apoptosis/necrosis of HepG2 cells incubated with NK, R-NK and G-NK cells at various of E/T ratio, n = 3. E–H The cytokine secretion from NK, R-NK and G-NK cells after co-incubating with HepG2 cells determined by ELISA analysis. Statistical analysis was performed by two-tailed paired sample Student’s t-tests (*p < 0.05, **p < 0.01, ***p < 0.001), n = 3