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. 2023 Sep 4;14:5364. doi: 10.1038/s41467-023-40870-1

Fig. 3. Schematics of bioprotonic devices with biotin-Streptavidin and aptamer-peptide.

Fig. 3

a i) 2-cholesterol handled DNA nanopores with biotin in the absence of streptavidin (6HB-2C-2B). Created pathway facilitates H+ transfer without inhibition of binding due to absence of streptavidin; ii) 2-cholesterol-handled DNA nanopores with binding of biotin-streptavidin (6HB-2C-2B/S-avidin). H+ transfer is inhibited by blocked pore channels; iii) 2-cholesterol-handled DNA nanopores without biotin in the presence of streptavidin (6HB-2C/S-avidin). The pores are not blocked by binding due to lacking biotin. b IH+ versus time plot for V = −400 mV. Orange trace 6HB-2C-2B (3a-i), green trace 6HB-2C-2B/S-avidin (3a-ii) and blue trace 6HB-2C/S-avidin (3a-iii). We measured IH+ = −96 ± 21 nA, −12 ± 6 nA and −92 ± 9 nA with 6HB-2C-2B, 6HB-2C-2B/S-avidin and 6HB-2C/S-avidin, respectively. Error bars are 1 s.d. (n = 3). c i) 2-cholesterol handled DNA nanopores with SELEX based DNA aptamer in the absence of B-type natriuretic peptide (6HB-2C-2AP). Created pathway facilitates H+ transfer without inhibition of binding due to absence of peptide; ii) 2-cholesterol-handled DNA nanopores with binding of aptamer-peptide (6HB-2C-2AP/BNP). H+ transfer is slightly inhibited by blocked pore channels; iii) 2-cholesterol-handled DNA nanopores without aptamer in the presence of peptide (6HB-2C/BNP). d IH+ versus time plot for V = −400 mV. Orange trace 6HB-2C-2AP (3c-i), green trace 6HB-2C-2AP/BNP (3c-ii) and blue trace 6HB-2C/BNP (3c-iii). We measured IH+ = −90 ± 3 nA, −51 ± 1 nA and −96 ± 9 nA with 6HB-2C-2AP, 6HB-2C-2AP/BNP and 6HB-2C/BNP, respectively. Error bars are 1 s.d. (n = 3).