Figure 2.

Isotropic, uniform random sections of the rat testes were obtained by applying the orientator method. (A) The testis for each animal was embedded in an agar block and placed at the center of a circle with 90 equidistant divisions along the perimeter. A random number between 0 and 90 was chosen. (B) The agar medium was cut along a parallel line according to the direction of the selected number (here, 35). (C) The agar block was placed on its cut surface at the center of a second circle, with 96 non-equidistant divisions along its perimeter. The agar was cut into multiple equidistant pieces along a parallel line according to the direction of a random number from 0 to 96 (here, 50) using a tissue slicer.