Figure 2.
Experimental design for serial polar body (PB) transfer between two oocytes, ×200 magnification. (A) Matured oocyte with a visible meiotic spindle (by PolScope, OCTAX PolarAID; OCTAX) from first PB nuclear transfer (PB1NT) group (arrow 1: meiotic spindle; arrow 2: PB). (B) Piercing the zona with a laser (OCTAX Laser Shot; MTG) and removing the PB1 oocyte. (C) Isolation of the meiotic spindle (arrow). (B) Biopsy of PB1 from another oocyte in PB1NT and using it as a genome donor. (E) Donor PB1 placed in hemagglutinating virus of Japan Envelope Vector. (F) Donor PB1 placed in the sub-zona environment.