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. 2023 Aug 22;13:1169101. doi: 10.3389/fcimb.2023.1169101

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Copyright © 2023 Hou, Zhang, Jiang, Yang, Liu, Yuan, Sun and Liu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alpha and beta diversity of the raw and decontaminated 2bRAD sequencing data. (A–F) Species richness and Shannon index of the raw data and decontaminated data were calculated separately to evaluate the alpha diversity of NBM and non-NBM individuals from different body sites (blood, CSF, and skin). (G–I) Beta diversity was implemented using the Bray-Curtis distance-based PCoA. Both species richness and Shannon index showed that this method of contamination removal did not significantly impact the comparisons of the alpha diversity of NBM and non-NBM individuals (p > 0.05).