Table 3.
Slope (m), intercept (c), sensitivity, affinity, ED50, and R² of prednisolone assays, using PSL-21-HS-BSA-antibody and PSL-21-HS-HRP (with spacers) enzyme conjugates.
| Assay combination of PSL-21-HS-BSA- antibody with | Slope (m) and intercept (c) | Sensitivity of the assay (ng/mL) | Affinity of the assay (mole/L) |
ED50 (ng/mL) | R² | |
|---|---|---|---|---|---|---|
| m | c | |||||
| PSL-21-HS-ADH-HRP Enzyme conjugate |
-2.47 | 2.06 | 1.22 | 1.18 x 10-7 | 6.79 | 0.9 |
| PSL-21-HS-CH-HRP Enzyme conjugate |
-1.80 | 1.87 | 0.59 | 5.07 x 10-8 | 4.03 | 1.0 |
| PSL-21-HS-EDA-HRP Enzyme conjugate |
-2.15 | 1.61 | 0.48 | 4.71 x 10-8 | 3.60 | 1.0 |
| PSL-21-HS-U-HRP Enzyme conjugate |
-1.84 | 1.03 | 0.018 | 5.09 x 10-8 | 1.89 | 1.0 |