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Recovery of prednisolone from exogenously spiked human serum pools using the PSL-21-HS-BSA-antibody and PSL-21-HS-Urea-HRP-enzyme conjugate.
| Human serum pools | Prednisolone added (ng/mL) | Prednisolone observed (ng/mL) | Prednisolone expected (ng/mL) | % Recovery |
|---|---|---|---|---|
| Pool A (Basal) | – | 0.75 | –– | – |
| Pool B | 0.62 | 1.21 | 1.37 | 88.32 |
| Pool C | 1.25 | 2.05 | 2.00 | 102.50 |
| Pool D | 5.0 | 5.55 | 5.75 | 98.26 |
| Pool E | 15.0 | `15.50 | 15.75 | 98.41 |
| Pool F | 25.0 | 25.80 | 25.75 | 100.19 |
